Protein synthesis in hepatocytes isolated from patients with gastrointestinal malignancy.
نویسندگان
چکیده
منابع مشابه
Protein synthesis in hepatocytes isolated from patients with gastrointestinal malignancy.
To investigate the effect of remote and proximate cancer on hepatic protein metabolism, we determined rates of total protein synthesis by hepatocytes (HPS) isolated from 31 patients undergoing liver wedge biopsy: 7 patients with benign disease, 14 with gastric cancer, and 10 with colorectal cancer (5 of whom had liver metastases). Patients with malignant disease without weight loss had a threef...
متن کاملPlasma protein synthesis by isolated rat hepatocytes
A system of preparation of rat hepatocytes with extended viability has been developed to study the role of hormones and other plasma components upon secretory protein synthesis. Hepatocytes maintained in minimal essential medium reduced the levels of all amino acids in the medium except the slowly catabolized amino acids leucine, isoleucine, and valine, which steadily increase as the result of ...
متن کاملDiscrimination of Dominant H. pylori Strains Isolated from Patients with Different Gastroduodenal Pathologies by Protein Profiling
It is not clear what factors determine divergent outcomes of infections caused by H. pylori . In the present study, the protein profiles of different strains of H. pylori, isolated from three groups of patients with ulcerative disease, non-ulcerative gastritis and cancer disease, were analyzed using 1DSDS-PAGE. The patterns of different H. pylori strains were highly divergent. About 30.76% (7 b...
متن کاملEffects of amino acids, ammonia and leupeptin on protein synthesis and degradation in isolated rat hepatocytes.
Protein synthesis in isolated rat hepatocytes, as measured by the incorporation of [14C]-valine at constant specific radioactivity, proceeded at a rate of 0.3-0.5%/h in an unsupplemented medium, i.e. only about one-tenth the rate of protein degradation (4%/h). Leupeptin, which inhibits lysosomal protein degradation (previously found to be 75% of the total degradation in hepatocytes), had no eff...
متن کاملRegulation of glucose synthesis in hormone-sensitive isolated rat hepatocytes.
A simplified procedure was developed for isolation of intact, hormone-sensitive liver cells in a high and reproducible yield. These cells produce glucose from various precursors at rates comparable to those achieved in isolated perfused liver. Glucagon enhanced glucose synthesis from pyruvate, dihydroxyacetone, fructose, or xylitol more effectively at low than at high substrate concentration. A...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1987
ISSN: 0021-9738
DOI: 10.1172/jci113216